Coding

Part:BBa_K2082208:Design

Designed by: Pascal Schmidt   Group: iGEM16_Bielefeld-CeBiTec   (2016-10-07)


Fusion protein Gal4:RpoZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 357
    Illegal BamHI site found at 105
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Jeong et al. (2001) wrote about the interaction of Gal4 and Gal11P.
The sequence for the modificated Gal11P was designed on the results of the publication by Himmelfarb et al. (1990)
The use of this proteins in a bacterial two-hybrid system was inspirated by Badran et al. (2016) and Joung et al. (2000).


Source

Sequence ordered by gene synthesis from IDT.

References

  • Badran, A. H., Guzov, V. M. & Huai, Q. et al. (2016) Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Nature 533 (7601), 58–63.
  • Himmelfarb, H. J., Pearlberg, J., Last, D. H. & Ptashne, M. (1990) GAL11P: a yeast mutation that potentiates the effect of weak GAL4-derived activators. Cell 63 (6), 1299–1309.
  • Jeong, C. J., Yang, S. H., Xie, Y., Zhang, L., Johnston, S. A. & Kodadek, T. (2001) Evidence that Gal11 protein is a target of the Gal4 activation domain in the mediator. Biochemistry 40 (31), 9421–9427.
  • Joung, J. K., Ramm, E. I. & Pabo, C. O. (2000) A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions. Proceedings of the National Academy of Sciences of the United States of America 97 (13), 7382–7387.